Since newborn screening efforts were facilitated by the use of Guthrie spots in 1963, the development of a routine procedure for microextraction of DNA and recently even RNA from these specimens would allow direct screening at the molecular
level.
The
ability to extract DNA and RNA from very small dried blood specimens will have an impact on newborn screening program and a mass screening test. The advantage of these dried blood specimens on Whatman filter papers includes simplicity of sample
acquisition, ease of sample transport, and stability of the analyte. Until now, the microextraction of each DNA, and RNA was carried out from each other method. However, we performed coincidentally the microextraction of DNA and RNA in the same
procedure and obtained the good results. These procedure is more time-saving, more convenient, and more economical than previous other method.
Therefore, we set up adequate pretreatment and analytic condition of molecular biology and provide the basic datas in the diagnosis and research of newborn screening test, genetic disease, infectious disease, autoimmune disease, and cancer, and
the
identification of individuals in forensic medicine.
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